Fungal species characterization associated with vanilla root rot in the Totonacapan, Veracruz, Mexico

Vanilla (Vanilla planifolia) is a crop of great biocultural importance in the Totonacapan region Mexico; however, producers face various phytosanitary problems that decrease production. The main one being loss caused by fungus, Fusarium oxysporum f. sp. vanillae, pathogen causes disease whose symptomatology root rot severe cases death plants. Therefore, study crucial, due to scarcity genetic variability vanilla, condition makes crops equally susceptible pathogen. present work aimed generate and molecularly pathogenically characterize fu-ngal isolates collection obtained from V. planifolia with rot. Roots were collected six municipalities region, for fungal isolation, semiselective culture medium was used, later, molecular characterization carried out through amplification sequencing partial translational elongation factor 1α gene (TEF1). pathogenic on vanilla leaves humid chamber, inoculating discs mycelium, making observations 18 days every 48 h. As results, 94 strains obtained, which 81 belong genus (42 F. strains), eight Colletotrichum, three Trichoderma two it not possible identify them, only 23 showed be different degrees virulence. results will crucial further studies comparative genomics oxysporum.